شبیه سازی عددی بیورآکتور غشایی بافت مجوف و بررسی پارامترهای مربوط به کشت سلول های حیوانی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 کارشناسی ارشد، دانشکده مهندسی مکانیک، دانشگاه صنعتی سهند، تبریز، ایران

2 استاد، دانشکده مهندسی مکانیک، دانشگاه صنعتی سهند، تبریز، ایران

3 استاد، دانشکده مهندسی شیمی، دانشگاه صنعتی سهند، تبریز، ایران

4 استادیار، دانشکده مهندسی شیمی، دانشگاه صنعتی سهند، تبریز، ایران

چکیده

در طی دهه­های اخیر توجه بسیاری از محققین به مهندسی بافت و سلول درمانی جلب شده است. بیورآکتورهای غشایی بافت مجوف یکی از روش­های مدرن تولید ساختارهای سه بعدی بافت زنده می­باشند. در مقاله حاضر، به شبیه‌سازی چهار رشته بافت مجوف در کنار یکدیگر با استفاده از بسته‌ نرم‌افزاریANSYS FLUENT پرداخته­ شده و اثرات همه آنها، بر روی محیط سلول‌ها به­صورت سه­بعدی مورد مطالعه قرارگرفته­است. برای پیش­بینی و طراحی بهینه بیورآکتور غشایی، پارامترهای جریان و توزیع غلظت‌های اجزای مهم شامل اکسیژن و گلوکز محاسبه و بررسی شده­اند. نتایج حاصل از بررسی تاثیر غلظت سلولی و فاصله از ورودی بر روی غلظت­های اکسیژن و گلوکز نشان داد که در غلظت­های سلولی بالا (2 cells/ml)، با افزایش فاصله از ورودی، میزان غلظت اکسیژن و گلوکز کاهش چشمگیری پیدا می­کند. همچنین با افزایش سرعت ورودی، افزایش چشمگیری در میزان غلظت‌ اکسیژن و گلوکز مشاهده شد. نتایج مربوط به بررسی تغییرات طول بیورآکتور نشان داد که غلظت اکسیژن و گلوکز در طول رشته­ها و در فواصل مختلف از ورودی، تغییر نکردند.

کلیدواژه‌ها

موضوعات


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